Cytotoxic and antiviral activity of extracts and compounds isolated from Clusia fluminensis Planch. & Triana (Clusiaceae)

Authors

  • Izabel Christina Nunes de Palmer Paixão Universidade Federal Fluminense
  • Levino da Costa Meneses Universidade Federal Fluminense
  • Helena de Souza Pereira Universidade Federal Fluminense
  • Maria Carolina Anholeti Universidade Federal Fluminense; Universidade Federal do Rio de Janeiro
  • Maria Raquel Figueiredo Instituto de Tecnologia em Fármacos
  • Maria Auxiliadora Coelho Kaplan Universidade Federal do Rio de Janeiro
  • Marcelo Guerra Santos Universidade do Estado do Rio de Janeiro
  • Selma Ribeiro de Paiva

Keywords:

Clusia fluminensis, Clusiaceae, cytotoxic activity, antiviral activity, HSV-1, HIV-1 RT, lanosterol

Abstract

Introduction: The worldwide distribution of herpes simplex virus type 1 (HSV-1) allied to the emergence of resistant strains makes necessary and urgent the search and development of new substances capable of preventing and treating HSV-1 infections. Studies demonstrate synergy between genital herpes and human immunodeficiency virus type 1 (HIV-1), which represents a major concern for global public health. Objective: The objective of this study was to evaluate the activity of crude extracts and isolated substances from C. fluminensis in the in vitro replication of the HSV-1 virus and HIV-1-RT activity. Methods: This study evaluated the activity of extracts and isolated compounds from Clusia fluminensis Planch. & Triana against HSV-1 using Vero cells in culture and against HIV-1 using a recombinant reverse transcriptase enzyme (HIV -1 RT). The percentage of inhibition against HSV-1 was determined from viral lysis plaque reduction assay, and the anti-HIV-1-RT test was performed by a fluorimetric assay. It was also evaluated the cytotoxic activity of the samples using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. Results: The crude extracts showed high percentage of inhibition against HSV-1, reaching 81.4 to 100.0% inhibition in non-cytotoxic concentration (50 μg/mL). The isolated compounds, lanosterol and clusianone, demonstrated 100% inhibition in non-cytotoxic concentration (50 μg/mL). We also examined the effects of the extracts and isolates on the activity of the HIV-1-RT. Among the crude extracts, only the methanolic extract of leaves and methanolic extract of stems showed inhibitory activity against HIV-1-RT. Regarding the isolated compounds, lanosterol showed a moderate activity. Conclusion: Our data demonstrate that extracts and isolates compounds Clusia fluminensis Planch. & Triana have promising antiviral activity inhibiting HSV-1 replication and HIV-1 by inhibiting the anti-RT activity.

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Author Biographies

Izabel Christina Nunes de Palmer Paixão, Universidade Federal Fluminense

Laboratório de Virologia Molecular, Universidade Federal Fluminense (UFF) – Niterói (RJ), Brasil

Levino da Costa Meneses, Universidade Federal Fluminense

Laboratório de Virologia Molecular, Universidade Federal Fluminense (UFF) – Niterói (RJ), Brasil

Helena de Souza Pereira, Universidade Federal Fluminense

Laboratório de Virologia Molecular, Universidade Federal Fluminense (UFF) – Niterói (RJ), Brasil

Maria Carolina Anholeti, Universidade Federal Fluminense; Universidade Federal do Rio de Janeiro

Laboratório de Botânica Estrutural e Funcional, UFF – Niterói (RJ), Brasil. Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ) – Rio de Janeiro (RJ), Brasil

Maria Raquel Figueiredo, Instituto de Tecnologia em Fármacos

Laboratório de Química de Produtos Naturais, Instituto de Tecnologia em Fármacos (Farmanguinhos)/Fundação Oswaldo Cruz – Rio de Janeiro (RJ), Brasil

Maria Auxiliadora Coelho Kaplan, Universidade Federal do Rio de Janeiro

Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ) – Rio de Janeiro (RJ), Brasil

Marcelo Guerra Santos, Universidade do Estado do Rio de Janeiro

Departamento de Ciências, Faculdade de Formação de Professores, Universidade do Estado do Rio de Janeiro (UERJ) – Rio de Janeiro (RJ), Brasil.

Selma Ribeiro de Paiva

Laboratório de Botânica Estrutural e Funcional, UFF – Niterói (RJ), Brasil

Published

2022-04-20

How to Cite

Paixão, I. C. N. de P., Meneses, L. da C., Pereira, H. de S., Anholeti, M. C., Figueiredo, M. R., Kaplan, M. A. C., Santos, M. G., & Paiva, S. R. de. (2022). Cytotoxic and antiviral activity of extracts and compounds isolated from Clusia fluminensis Planch. & Triana (Clusiaceae). Brazilian Journal of Sexually Transmitted Diseases, 27(3-4), 73–78. Retrieved from https://bjstd.org/revista/article/view/736

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Original Article